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mouse anti-human dcir  (Dendritics)

 
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    Structured Review

    Dendritics mouse anti-human dcir
    (A–B) Confocal images of dendritic cells cultured on glass (A) and on filters with 1 μm pore size (B). Actin was labeled with phalloidin-Alexa fluor 546 (Phal; magenta) and clathrin was visualized by immunostaining (AB; green). The filters were impregnated with Alexa fluor 633-labeled gelatin (Filter; grey). The yellow line indicates the position of the orthogonal view. Yellow arrow heads indicate randomly selected actin-rich cores. The red arrow head indicates the approximate filter surface. (C) Quantification of the fraction of clathrin positive actin cores from panels A–B. (D–F) Same as panels A–C, but now with immunostaining <t>for</t> <t>DC-SIGN,</t> <t>DCIR,</t> Dectin-1, CD206 and CD71. Error bars show the spread of data for multiple cells from at least two independent experiments. Scale bars, 2 μm.
    Mouse Anti Human Dcir, supplied by Dendritics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti-human dcir/product/Dendritics
    Average 90 stars, based on 1 article reviews
    mouse anti-human dcir - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "Podosomes of dendritic cells facilitate antigen sampling"

    Article Title: Podosomes of dendritic cells facilitate antigen sampling

    Journal: Journal of cell science

    doi: 10.1242/jcs.141226

    (A–B) Confocal images of dendritic cells cultured on glass (A) and on filters with 1 μm pore size (B). Actin was labeled with phalloidin-Alexa fluor 546 (Phal; magenta) and clathrin was visualized by immunostaining (AB; green). The filters were impregnated with Alexa fluor 633-labeled gelatin (Filter; grey). The yellow line indicates the position of the orthogonal view. Yellow arrow heads indicate randomly selected actin-rich cores. The red arrow head indicates the approximate filter surface. (C) Quantification of the fraction of clathrin positive actin cores from panels A–B. (D–F) Same as panels A–C, but now with immunostaining for DC-SIGN, DCIR, Dectin-1, CD206 and CD71. Error bars show the spread of data for multiple cells from at least two independent experiments. Scale bars, 2 μm.
    Figure Legend Snippet: (A–B) Confocal images of dendritic cells cultured on glass (A) and on filters with 1 μm pore size (B). Actin was labeled with phalloidin-Alexa fluor 546 (Phal; magenta) and clathrin was visualized by immunostaining (AB; green). The filters were impregnated with Alexa fluor 633-labeled gelatin (Filter; grey). The yellow line indicates the position of the orthogonal view. Yellow arrow heads indicate randomly selected actin-rich cores. The red arrow head indicates the approximate filter surface. (C) Quantification of the fraction of clathrin positive actin cores from panels A–B. (D–F) Same as panels A–C, but now with immunostaining for DC-SIGN, DCIR, Dectin-1, CD206 and CD71. Error bars show the spread of data for multiple cells from at least two independent experiments. Scale bars, 2 μm.

    Techniques Used: Cell Culture, Labeling, Immunostaining



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    (A–B) Confocal images of dendritic cells cultured on glass (A) and on filters with 1 μm pore size (B). Actin was labeled with phalloidin-Alexa fluor 546 (Phal; magenta) and clathrin was visualized by immunostaining (AB; green). The filters were impregnated with Alexa fluor 633-labeled gelatin (Filter; grey). The yellow line indicates the position of the orthogonal view. Yellow arrow heads indicate randomly selected actin-rich cores. The red arrow head indicates the approximate filter surface. (C) Quantification of the fraction of clathrin positive actin cores from panels A–B. (D–F) Same as panels A–C, but now with immunostaining <t>for</t> <t>DC-SIGN,</t> <t>DCIR,</t> Dectin-1, CD206 and CD71. Error bars show the spread of data for multiple cells from at least two independent experiments. Scale bars, 2 μm.
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    (A–B) Confocal images of dendritic cells cultured on glass (A) and on filters with 1 μm pore size (B). Actin was labeled with phalloidin-Alexa fluor 546 (Phal; magenta) and clathrin was visualized by immunostaining (AB; green). The filters were impregnated with Alexa fluor 633-labeled gelatin (Filter; grey). The yellow line indicates the position of the orthogonal view. Yellow arrow heads indicate randomly selected actin-rich cores. The red arrow head indicates the approximate filter surface. (C) Quantification of the fraction of clathrin positive actin cores from panels A–B. (D–F) Same as panels A–C, but now with immunostaining <t>for</t> <t>DC-SIGN,</t> <t>DCIR,</t> Dectin-1, CD206 and CD71. Error bars show the spread of data for multiple cells from at least two independent experiments. Scale bars, 2 μm.
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    Image Search Results


    (A–B) Confocal images of dendritic cells cultured on glass (A) and on filters with 1 μm pore size (B). Actin was labeled with phalloidin-Alexa fluor 546 (Phal; magenta) and clathrin was visualized by immunostaining (AB; green). The filters were impregnated with Alexa fluor 633-labeled gelatin (Filter; grey). The yellow line indicates the position of the orthogonal view. Yellow arrow heads indicate randomly selected actin-rich cores. The red arrow head indicates the approximate filter surface. (C) Quantification of the fraction of clathrin positive actin cores from panels A–B. (D–F) Same as panels A–C, but now with immunostaining for DC-SIGN, DCIR, Dectin-1, CD206 and CD71. Error bars show the spread of data for multiple cells from at least two independent experiments. Scale bars, 2 μm.

    Journal: Journal of cell science

    Article Title: Podosomes of dendritic cells facilitate antigen sampling

    doi: 10.1242/jcs.141226

    Figure Lengend Snippet: (A–B) Confocal images of dendritic cells cultured on glass (A) and on filters with 1 μm pore size (B). Actin was labeled with phalloidin-Alexa fluor 546 (Phal; magenta) and clathrin was visualized by immunostaining (AB; green). The filters were impregnated with Alexa fluor 633-labeled gelatin (Filter; grey). The yellow line indicates the position of the orthogonal view. Yellow arrow heads indicate randomly selected actin-rich cores. The red arrow head indicates the approximate filter surface. (C) Quantification of the fraction of clathrin positive actin cores from panels A–B. (D–F) Same as panels A–C, but now with immunostaining for DC-SIGN, DCIR, Dectin-1, CD206 and CD71. Error bars show the spread of data for multiple cells from at least two independent experiments. Scale bars, 2 μm.

    Article Snippet: The following primary antibodies were used for the immunofluorescence: mouse anti-vinculin (V9131, Sigma) at 1:200 dilution (v/v), mouse anti-talin (T3287, Sigma) at 1:100 (v/v), mouse anti-paxillin (349 | MAB3060, Sigma) at 1:100 (v/v), mouse anti-CD11b (ITGAM or Bear-1) (IM2581, Coulter) at 1:200 (v/v), mouse anti-CD29 (or ITGB1) (clone ts2/16) at 1:200 (v/v), mouse anti-MMP-14 (MAB3328, Bio Connect / MilliPore) at 1:100 (v/v), rat anti-tubulin (ab6161, Abcam) at 1:500 (v/v), mouse anti-human CD209 (or DC-SIGN) (551186, BD Bioscience) at 1:200 (v/v), mouse anti-human DCIR (DDX0180, Dendritics) at 1:200 (v/v), mouse anti-human Dectin-1 (MAB1859, R&D Systems) at 1:100 (v/v), mouse anti-human CD206 (555953, BD Biosciences) at 1:250 (v/v), mouse anti-human CD71 (347510, SALK) at 1:50 (v/v), mouse anti-clathrin light chain (C1985, Sigma) at 1:100 (v/v) and mouse anti-human MHC class II (clone Q5/13 ( Quaranta et al., 1980 )) at 20 μg ml −1 .

    Techniques: Cell Culture, Labeling, Immunostaining